Episkin CreamCream Episkin
In addition, one of the 5 fabrics is examined for history (H&E staining) and one for fabric vitality. To quantify this, the 3 rest of the fabrics are used for quantifying the melanin. Apply 1 ?l of the test specimen and 1 ?l of the test specimen (Melanex Duo; laboratory expansciences) each day to the strip of five times larger specimen size, skin ethicTM FEPE (size 0.5 cm²).
Therefore, all crops (including five times virgin tissue) are incubated at 37°C, 5% CO². The analyses (visual assessment, histology, MTT and extract melanin) are carried out at the end of the session. Note: This report is used for a cream or gel-formulated preparation; if the test preparation is a fluid, the report may be modified; it is also possible to test a preparation by systematic use in the agar.
Apply double culture to 300 microlitres of 0,5 mg/ml MTT for each test conditions and incubate 5% CO at 37 C for 3 hrs. Extract in 1,5 ml of isopropyl alcohol at room temperatures for at least 2 hrs. The results are presented as a percent of vitality in comparison to negativ control: Percent Vitality = [OD (570nm) test connection / OD (570nm) negativ control] x 100.
Repetitive application of the cream reduces the tan (B) in comparison to the normal tan (A). With Fontana Masson we find a connection between decreased pigments and lower levels of pigment in the melt. By the end of the test phase, all tissue (untreated, positively controlled and tested ) is captured with a photographic imager for visually assessing it.
By the end of the test phase, the 3 fabrics are taken out of the tray by removing the strainer with a knife. Measure the optical density on 80 microlitres of extracts at 490 nm using artificial Melanin as a benchmark. The results are given in OD and in mg/ml molanin.